Development of breeding lines by zigotic ovule culture in Nigella sativa L.

This study was carried out in order to develop an embryo culture technique and establish breeding lines with this technique. Ovule preferred the because seeds were too small isolation difficult. Embryo cultures done by using populations obtained from producers Samsun, Denizli, Isparta, Mersin Turkey ?ameli black cumin variety. Hybridizations according semi-diallel hybridization method gain zygotic embryos. LS2.5 MS (Sigma Aldrich Catalog number: M5519) mediums used for ovule MSD4 medium growing plants ovules. As a result of research, total 2904 ovules cultured medium. 148 them showed callus development; formation rate 5.10%. The highest investigated combinations x Denizli combination at 7.26%. Plant regeneration could not be these calluses. A 3526 Sixty plantlets plant determined as 1.70%. 41 matured harvested.